Biotinylated Mouse latent GDF-8 Protein, Avitag™,His Tag (pro & latent) 

AIDD‑Ready
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Menge
GD8-M82Q3-25ug
$360.00
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GD8-M82Q3-200ug
$1,420.00
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GD8-M82Q3
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Synonyms

GDF-8, Myostatin, GDF8, MSTN, Growth, differentiation factor 8

Source

Biotinylated Mouse latent GDF-8 Protein, Avitag,His Tag (GD8-M82Q3) is expressed from human 293 cells (HEK293). It contains AA Asn 25 - Ser 376 (Accession # O08689).

Predicted N-terminus: Gly

Molecular Characterization

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 43.7 kDa. The protein migrates as 40 kDa and 50-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background Introduction

Growth differentiation factor 8 (GDF8), also known as myostatin, is a unique member of the transforming growth factor-β superfamily that is expressed in human granulosa cells and has important roles in regulating a variety of ovarian functions. GDF8 acts as a negative regulator of skeletal muscle growth and differentiation. In addition to the expression in the musculoskeletal system, GDF8 is also expressed in various tissues, including the reproductive system.