Collect Product
Product Details
Synonyms
FCGR3A, CD16A, FCG3, FCGR3, IGFR3
Source
Biotinylated Human CD16a (F176), Avitag,His Tag (CDA-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1).
Predicted N-terminus: Gly 17
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 25.5 kDa. The protein migrates as 40-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
Kunden haben auch angesehen
Data Display
SDS-PAGE
Biotinylated Human CD16a (F176), Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) is more than 90% and the molecular weight of this protein is around 35-54 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 1.72 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Immobilized Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) on SA Chip can bind Rituximab with an affinity constant of 1.33 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) on SA Biosensor, can bind Rituximab with an affinity constant of 1.29 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
J***n2023-06-24
LikesWe used the biotinylated human Fc gamma RIIIA material from Acro to test the binding of different human antibodies. The material arrived quickly and in time for our experiments. I used the material with the Biacore 8K (SPR) instrument using Series S Biotin CAP chip. We were happy with how the material worked in this assay format. Overall, we were pleased with the quality and would recommend this to other researchers.
- D***g2022-12-07 LikesI have been using this product for screening antibody binding. The product showed satisfactory binding to streptavidin-coated beads, as well as satisfactory detection of an antibody control. I have also used a range of other biotinylated Fc gamma receptor products from Acro, which showed good binding to respective control antibodies, as well as stability at 4C when reconstituted (no compromised antibody binding results in a month).
Background Introduction
CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibodydependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
Frontier Progress
Drug Development Progress- English Name：
Low affinity immunoglobulin gamma Fc region receptor III-A
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
14 Details
- Highest R&D Stage：
Phase 2 Clinical
Contact Us
Drug Candidate Licensing
