Product Details
Synonyms
SLC3A2, CD98, 4F2, 4F2HC, 4T2HC, CD98HC, MDU1, NACAE
Source
Biotinylated Mouse CD98 Protein, Avitag,His Tag (CD8-M82Q3) is expressed from human 293 cells (HEK293). It contains AA Arg 100 - Ala 526 (Accession # P10852-1).
Predicted N-terminus: Gly
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 51.4 kDa. The protein migrates as 60-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE

Biotinylated Mouse CD98 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Biotinylated Mouse CD98 Protein, Avitag,His Tag (Cat. No. CD8-M82Q3) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Biotinylated Mouse CD98 Protein, Avitag,His Tag (Cat. No. CD8-M82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Purified anti-mouse CD98 (4F2) Antibody, Rat IgG2a with a linear range of 0.02-2 ng/mL (QC tested).
Protocol
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