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Biotinylated Mouse CD98 Protein, Avitag™,His Tag (MALS verified)

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Product Number/Spec
Price
Menge
CD8-M82Q3-25ug
$360.00
CD8-M82Q3-200ug
$1790.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    SLC3A2, CD98, 4F2, 4F2HC, 4T2HC, CD98HC, MDU1, NACAE

  • Source

    Biotinylated Mouse CD98 Protein, Avitag,His Tag (CD8-M82Q3) is expressed from human 293 cells (HEK293). It contains AA Arg 100 - Ala 526 (Accession # P10852-1).

    Predicted N-terminus: Gly

    Request for sequence
  • Molecular Characterization

    CD98 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 51.4 kDa. The protein migrates as 60-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    CD98 SDS-PAGE

    Biotinylated Mouse CD98 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD98 SEC-MALS

    The purity of Biotinylated Mouse CD98 Protein, Avitag,His Tag (Cat. No. CD8-M82Q3) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CD98 ELISA

    Immobilized Biotinylated Mouse CD98 Protein, Avitag,His Tag (Cat. No. CD8-M82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Purified anti-mouse CD98 (4F2) Antibody, Rat IgG2a with a linear range of 0.02-2 ng/mL (QC tested).

    Protocol

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Publish Comment

Background Introduction

CD antigen CD98 is also known as 4F2 cell-surface antigen heavy chain (4F2hc), 4F2 heavy chain antigen, Solute carrier family 3 member 2 (SLC3A2), Lymphocyte activation antigen 4F2 large subunit, is a single-pass type I I membrane protein which belongs to the SLC3A transporter family.. SLC3A2 / CD98 is expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. SLC3A2 / CD98 is required for the function of light chain amino-acid transporters and also involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. CD98 involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A5 or SLC7A8, CD98 involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals.

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