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CD4, CD4mut, LEU3
Biotinylated Human CD4, Fc,Avitag (CD4-H82F3) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # P01730-1).
Predicted N-terminus: Lys 26
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 69.7 kDa. The protein migrates as 90-100 kDa under reducing (R) condition, and > 180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Less than 0.2 EU per μg by the LAL method / rFC method.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.