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Biotinylated Human CD209 / DC-SIGN Protein, Avitag™,His Tag

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Product Number/Spec
Price
Menge
CD9-H82Q3-25ug
$360.00
CD9-H82Q3-200ug
$1565.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    DC-SIGN, CD209, DC-SIGN1, CLEC4L

  • Source

    Biotinylated Human CD209 Protein, Avitag,His Tag (CD9-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Gln 59 - Ala 404 (Accession # Q9NNX6-1).

    Predicted N-terminus: Gly

    Request for sequence
  • Molecular Characterization

    CD209 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 43.0 kDa. The protein migrates as 47-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    CD209 SDS-PAGE

    Biotinylated Human CD209 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     CD209 ELISA

    Immobilized SARS-CoV-2 Spike S1, His Tag (Cat. No. S1N-C52Ht) at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD209 Protein, Avitag,His Tag (Cat. No. CD9-H82Q3) with a linear range of 0.07-0.313 μg/mL (QC tested).

    Protocol

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Publish Comment

Background Introduction

CD209 is also known as CLEC4L, DC-SIGN and CD209 antigen. Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.

Frontier Progress

 
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