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Product Details
Synonyms
CD19, B4, CVID3, MGC12802
Source
Biotinylated Human CD19 Protein, Fc Tag, primary amine labeling (CD9-H8259) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1). It is the biotinylated form of Human CD19 (20-291), Fc Tag (Cat. No. CD9-H5259).
Predicted N-terminus: Pro 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 56.3 kDa. The protein migrates as 60-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Human CD19 Protein, Fc Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized FMC63 at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD19 Protein, Fc Tag, primary amine labeling (Cat. No. CD9-H8259) with a linear range of 0.039-0.625 μg/mL (QC tested).
Protocol
Immobilized a series of concentration of Biotinylated Human CD19 Protein, Fc Tag, primary amine labeling (Cat. No. CD9-H8259) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind FMC63 (Mouse IgG2a) 2 μg/mL (100 μL/well) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
Protocol
User Reviews Publish Comment
1***22019-10-22
LikesThe biotin-labeled CD19 protein was purchased to test the positive expression rate of CAR-T cells. According to the recommended concentration, we detected good positive CAR-T cells after incubation, and the effect was good.
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
B-lymphocyte antigen CD19
- Category：
- Listed Drugs Count：
14 Details
- Clinical Drugs Count：
435 Details
- Highest R&D Stage：
Approved
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