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Biotinylated Human ANGPTL7 / CDT6 Protein, Mouse IgG2a Fc,Avitag™

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Product Number/Spec
Price
Menge
AN7-H82F3-25ug
$360.00
AN7-H82F3-200ug (100ug X 2)
$1565.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    ANGPTL7, CDT6

  • Source

    Biotinylated Human ANGPTL7, Mouse IgG2a Fc,Avitag (AN7-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Pro 346 (Accession # O43827-1).

    Predicted N-terminus: Gln 27

    Request for sequence
  • Molecular Characterization

    ANGPTL7 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 65.8 kDa. The protein migrates as 60 kDa and 70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    ANGPTL7 SDS-PAGE

    Biotinylated Human ANGPTL7, Mouse IgG2a Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     ANGPTL7 ELISA

    Immobilized Human LILRB2, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc,Avitag (Cat. No. AN7-H82F3) with a linear range of 0.156-2.5 μg/mL (QC tested).

    Protocol

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Publish Comment

Background Introduction

Angiopoietin-like 7 (ANGPTL7) is also known as Corneal-Derived Transcript 6 (CDT6), and is a secreted glycoprotein that is structurally related to the angiopoietins. It is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells.

Frontier Progress

 
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