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Product Details
Synonyms
ANGPT2, AGPT2, ANG2, Angiopoietin-2
Source
Biotinylated Human Angiopoietin-2 Protein, His,Avitag (AN2-H82E9) is expressed from human 293 cells (HEK293). It contains AA Tyr 19 - Phe 496 (Accession # O15123-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 58.6 kDa. The protein migrates as 60-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 150 mM NaCl, 5 mM CHAPS, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Biotinylated Human Angiopoietin-2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human TIE2, Fc Tag (Cat. No. TI2-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Angiopoietin-2 Protein, His,Avitag (Cat. No. AN2-H82E9) with a linear range of 0.005-0.156 μg/mL (QC tested).
Protocol
Immobilized Biotinylated Human Angiopoietin-2 Protein, His,Avitag (Cat. No. AN2-H82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human TIE2, Fc Tag (Cat. No. TI2-H5255) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human TIE2, Fc Tag (Cat. No. TI2-H5255) captured on Protein A Chip can bind Biotinylated Human Angiopoietin-2 Protein, His,Avitag (Cat. No. AN2-H82E9) with an affinity constant of 58.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Angiopoietin-2
- Category：
- Listed Drugs Count：
1 Details
- Clinical Drugs Count：
13 Details
- Highest R&D Stage：
Approved
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