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Product Details
Synonyms
Streptavidin, SA
Source
Streptavidin Protein-Cy3 (STN-NC114) is expressed from E. coli cells.
Predicted N-terminus: Met
Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 13.8 kDa.
Conjugate
Cy3
Excitation Wavelength: 555 nm
Emission Wavelength: 569 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with Cy3 using standard chemical labeling method. The residual Cy3 is removed by molecular sieve treatment during purification process.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
Bioactivity-Flow Cytometry
5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 ug/mL Biotinylated Human CD19 (20-291) Protein, Fc,Avitag™, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-CY3 (Cat. No. STN-NC114) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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