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Human TWEAK Protein, Fc Tag (SPR verified)

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Product Number/Spec
Price
Menge
TWK-H5263-100ug
$385.00
TWK-H5263-1mg
$2310.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    TNFSF12

  • Source

    Human TWEAK Protein, Fc Tag (TWK-H5263) is expressed from human 293 cells (HEK293). It contains AA Ser 94 - His 249 (Accession # O43508-1).

    Predicted N-terminus: Pro

    Request for sequence
  • Molecular Characterization

    TWEAK Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 43.6 kDa. The protein migrates as 33 kDa and 48-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    TWEAK SDS-PAGE

    Human TWEAK Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-SPR

     TWEAK SPR

    Human TWEAK Protein, Fc Tag (Cat. No. TWK-H5263) immobilized on CM5 Chip can bind Human TWEAK R, Fc Tag (Cat. No. TNA-H526a) with an affinity constant of 42.8 pM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Publish Comment

Background Introduction

TWEAK (tumor necrosis factor-like weak inducer of apoptosis) is a member of the TNF superfamily that controls a multitude of cellular events including proliferation, migration, differentiation, apoptosis, angiogenesis, and inflammation. TWEAK exists in both membrane-bound and secreted forms, and can induce cell apoptosis via multiple pathways of cell death in a cell type-specific manner. It has also been found to promote the proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.

Frontier Progress

 
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