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Product Details
Synonyms
TSLP R, CRLF2, IL-XR, ILXR, p2RY8, CRLF2 fusion, TSLP receptor, TSLPR, CRL2, CRLF2Y, Cytokine receptor-like 2
Source
Human TSLP R, Fc Tag (TSR-H525a) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Lys 231 (Accession # Q9HC73-1).
Predicted N-terminus: Gly 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 50.4 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human TSLP R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TSLP R, Fc Tag (Cat. No. TSR-H525a) is more than 90% and the molecular weight of this protein is around 115-140 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with a linear range of 4-31 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) at 2 μg/mL (100 μL/well) via precoated 5 μg/mL (100 μL/well) of Human TSLP R, Fc Tag (Cat. No. TSR-H525a), can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) with a linear range of 1-39 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Captured Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 1.81 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
Captured Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 4.22 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) with an affinity constant of 16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) with an affinity constant of 24.7 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).
Protocol
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Human TSLP Protein (R127A, R130A), His Tag (Cat. No. TSP-H5243) with an affinity constant of 23 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).
Protocol
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with an affinity constant of 13.2 nM as determined in BLI assay (ForteBio Octet R8)(Routinely tested).
Protocol
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Biotinylated Human TSLP (R127A, R130A) Protein, His,Avitag (Cat. No. TSP-H82E0) with an affinity constant of 9.67 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Thymic stromal lymphopoietin
- Category：
- Listed Drugs Count：
1 Details
- Clinical Drugs Count：
28 Details
- Highest R&D Stage：
Approved
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