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Product Details
Synonyms
TACSTD2, GA733-1, M1S1, TROP2
Source
Biotinylated Human TROP-2 (88-274), His,Avitag (TR2-H82Ea) is expressed from human 293 cells (HEK293). It contains AA Thr 88 - Thr 274 (Accession # P09758-1).
Predicted N-terminus: Thr 88
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 25.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Human TROP-2 (88-274), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human TROP-2 (88-274), His,Avitag (Cat. No. TR2-H82Ea) is more than 85% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human TROP-2 (88-274), His,Avitag (Cat. No. TR2-H82Ea) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Monoclonal Antibody Against Human TROP-2 Protein,Mouse IgG1 with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Tumor-associated calcium signal transducer 2
- Category：
- Listed Drugs Count：
3 Details
- Clinical Drugs Count：
47 Details
- Highest R&D Stage：
Approved
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