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Product Details
Synonyms
TL1A, VEGI, TNFSF15
Source
Human TL1A Protein, His Tag (TLA-H5244) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Leu 192 (Accession # O95150-2).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.7 kDa. The protein migrates as 30 kDa and 31-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human TL1A Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TL1A Protein, His Tag (Cat. No. TLA-H5244) is more than 95% and the molecular weight of this protein is around 80-110 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human DcR3, Fc Tag (Cat. No. TNB-H5255) at 5 μg/mL (100 μL/well) can bind Human TL1A, His Tag (Cat. No. TLA-H5244) with a linear range of 0.5-15 ng/mL (QC tested).
Protocol
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5244) at 5 μg/mL (100 μL/well) can bind Human DcR3, Fc Tag (Cat. No. TNB-H5255) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
Immobilized Human TL1A Protein, His Tag (Cat. No. TLA-H5244) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Monoclonal Anti-TL1A Antibody, Human IgG1 and 4 μg/mL (50 μL/well) Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a half maximal inhibitory concentration (lC50) of 3.06 μg/mL (Routinely tested).
Protocol
Immobilized Human TL1A Protein, His Tag (Cat. No. TLA-H5244) at 10 μg/mL (100 μL/well) can bind Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) with a linear range of 0.005-0.156 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-TL1A antibody captured on Protein A Chip can bind Human TL1A Protein, His Tag (Cat. No. TLA-H5244) with an affinity constant of 0.218 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Tumor necrosis factor superfamily member 15
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
18 Details
- Highest R&D Stage：
Phase 3 Clinical
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