Human Tenascin-C Protein, His Tag

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TEC-H52H3-100ug
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Synonyms

Tenascin C, TNC, FN3

Source

Human Tenascin-C Protein, His Tag (TEC-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 2 - Asp 254 (Accession # A0A994J5G0).

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 29.3 kDa. The protein migrates as 40-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Tenascin-C [Source: HGNC Symbol; Acc: HGNC: 5318] come from database entries Ensembl: ENSP00000516086.1. This transcript has 6 exons, is annotated with 15 domains and features, is associated with 12220 variant alleles and maps to 170 oligo probes.