Product Details
Synonyms
Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
Source
Bat SARS-like coronavirus WIV1 Spike RBD Protein, His Tag (SPD-S52Ha) is expressed from human 293 cells (HEK293). It contains AA Arg 307 - Gln 524 (Accession # AGZ48828.1).
Predicted N-terminus: Arg 307
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.4 kDa. The protein migrates as 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE

Bat SARS-like coronavirus WIV1 Spike RBD Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Bat SARS-like coronavirus WIV1 Spike RBD Protein, His Tag (Cat. No. SPD-S52Ha) is more than 90% and the molecular weight of this protein is around 28-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind Bat SARS-like coronavirus WIV1 Spike RBD Protein, His Tag (Cat. No. SPD-S52Ha) with a linear range of 0.001-0.125 μg/mL (QC tested).
Protocol
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