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Product Details
Synonyms
Spike, S protein, Spike glycoprotein, S glycoprotein
Source
SARS coronavirus SZ1 Spike Trimer Protein, His Tag (SPN-S52Hx) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAS00003.1 (HT74-75R, R667A, KV968-969PP, E1163K, L1179F)).
Predicted N-terminus: Ser 14
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 136.4 kDa. The protein migrates as 140-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
SARS coronavirus SZ1 Spike Trimer Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of SARS coronavirus SZ1 Spike Trimer Protein, His Tag (Cat. No. SPN-S52Hx) is more than 90% and the molecular weight of this protein is around 510-560 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS coronavirus SZ1 Spike Trimer Protein, His Tag (Cat. No. SPN-S52Hx) with a linear range of 0.004-0.125 μg/mL (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Coronavirus spike glycoprotein
- Category：
- Listed Drugs Count：
15 Details
- Clinical Drugs Count：
88 Details
- Highest R&D Stage：
Approved
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