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Product Details
Synonyms
SDF-1, CXCL12, hSDF-1, IRH, hIRH, PBSF, SDF1, SDF1A, SDF1B
Source
Human SDF-1, His Tag (SD1-H5143) is expressed from E. coli cells. It contains AA Lys 22 - Lys 89 (Accession # P48061-2).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 10.0 kDa. The protein migrates as 13-14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human SDF-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Human SDF-1, His Tag (Cat. No. SD1-H5143) was greater than 95% as determined by SEC-HPLC.
Bioactivity-SPR
Human CXCR4 Full Length Protein, Flag,His Tag (Cat. No. CX4-H52D3) immobilized on CM5 Chip can bind Human SDF-1, His Tag (Cat. No. SD1-H5143) with an affinity constant of 128 nM as determined in a SPR assay (in presence of Detergent Buffer B) (Biacore 8K) (QC tested).
Protocol
Bioactivity-BLI
Loaded Human SDF-1, His Tag (Cat. No. SD1-H5143) on NTA Biosensor, can bind Human CXCR4, Fc Tag (Cat. No. CX4-H5269) with an affinity constant of 41.7 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
C-X-C motif chemokine ligand 12
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
6 Details
- Highest R&D Stage：
Phase 2 Clinical
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