Human RANK / TNFRSF11A Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)

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RAK-H5251-100ug
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RAK-H5251-1mg (500ug X 2)
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RAK-H5251
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Synonyms

CD265, FEO, LOH18CR1, ODFR, OFE, OPTB7, OSTS, PDB2, RANK, TNFRSF11A, TRANCER, CD265 antigen, ODFROSTS, RANKLOH18CR1, NFKB activator

Source

Human RANK Protein, Mouse IgG2a Fc Tag (RAK-H5251) is expressed from human 293 cells (HEK293). It contains AA Ile 30 - Pro 212 (Accession # Q9Y6Q6-1).

Predicted N-terminus: Ile 30

Molecular Characterization

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 47.2 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background Introduction

RANK / TNFRSF11A is a member of the tumor necrosis factor receptor family. RANK / TNFRSF11A shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Functional genomics in mice showed that RANKL (TNFSF11), a member of the TNF ligand superfamily, and RANK (TNFRSF11a), the cognate TNFR family receptor for RANKL, were essential for osteoclastogenesis in vivo. A functional interaction between RANKL, expressed by bone stromal cells of the osteoblast lineage, and RANK, expressed by osteoclast precursors of hematopoietic myeloid lineage, is necessary for osteoclast differentiation, survival, and activation.