Human PTK7 / CCK4 Protein, His Tag, low endotoxin (Mass Spec & MALS verified)

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PT7-H52H3-100ug
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Synonyms

PTK7, CCK4, CCK-4, Tyrosine-protein kinase-like 7, Protein-tyrosine kinase 7, Protein-tyrosine kinase 7

Source

Human PTK7, His Tag (PT7-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 31 - Thr 704 (Accession # Q13308-1).

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 76.5 kDa. The protein migrates as 100-125 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Mass Spectrometry
  • Measured Molecular Mass

    The molecular weight by mass spectrometry is 76520 Da (Routinely tested).

  • Sequence Coverage

    Human PTK7, His Tag(PT7-H52H3) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage (Routinely tested).

  • N-terminal Sequence Analysis

    Ala‑Ile‑Val‑Ile‑Phe‑Ile‑Lys‑Gln‑Pro‑Ser‑Ser‑Gln‑Asp‑Ala‑Leu
    (Routinely tested)

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background Introduction

PTK7, highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.