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Product Details
Synonyms
FOLH1, PSMA, GIG27, FOLH, NAALAD1, PSM, NAALADase I, GCPII, FGCP
Source
Cynomolgus PSMA, His Tag (PSA-C5247) is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750 (Accession # A0A2K5VNZ0-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 81.4 kDa. The protein migrates as 100-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in MES and NaCl with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Data Display
SDS-PAGE
Cynomolgus PSMA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus PSMA, His Tag (Cat. No. PSA-C5247) is more than 85% and the molecular weight of this protein is around 200-245 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus PSMA, His Tag (Cat. No. PSA-C5247) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human PSMA Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
Bioactivity
Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >250 pmol/min/μg, as measured under the described conditions (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Prostate-Specific Membrane Antigen
- Category：
- Listed Drugs Count：
6 Details
- Clinical Drugs Count：
159 Details
- Highest R&D Stage：
Approved
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