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Prefusion glycoprotein F0/pre-F protein (RSV)
Human parainfluenza viruses Pre-fusion glycoprotein F0, His Tag (PR0-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Thr 493 (Accession # AGW51052.1).
Predicted N-terminus: Gln 19
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 57.8 kDa. The protein migrates as 58-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Within the expressed region, several mutations were introduced to optimize the protein expression and stability.
The protein is designed as a trimer.
Less than 1.0 EU per μg by the LAL method / rFC method.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.