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Product Details
Product Details
Phi29 DNA Polymerase is a DNA polymerase cloned from the Bacillus subtilis phage Phi29, expressed and purified in Escherichia coli. This enzyme exhibits remarkable strand displacement activity and high processivity, enabling it to synthesize DNA fragments of up to 70 kb in length. It also demonstrates strong template binding capability and possesses robust 3′→5′ exonuclease proofreading activity.
Materials Provided
ComponentsSizephi29 DNA polymerase250 U/1250 Uphi29 DNA Polymerase 10× Reaction Buffer1.5 mL1mg/mL BSA0.3 mL/1 mLApplication
Whole Genome Amplification (WGA).
Rolling Circle Amplification (RCA).
Long DNA Fragment Synthesis.Unit Definition
One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
Purity
>95% as determined by SDS-PAGE.
Enzyme Activity
>10 U/μL
Formulation
Supplied as 0.2 μm filtered solution in 10 mM Tris, pH7.4 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt;
- -20°C for 3 months under sterile conditions.
- *The 10× Reaction Buffer provided in this kit contains DTT, a sulfhydryl reducing agent essential for maintaining enzyme activity. When employing the 10× Reaction Buffer exposed to prolonged storage or repeated freeze-thaw cycles, fresh 40 mM DTT should be supplemented to the Reaction Buffer to ensure maximal enzymatic activity, as oxidative degradation of DTT progressively compromises its reducing capacity.
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Data Display
SDS-PAGE
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity
One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C (QC tested).
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