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PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Human PD-L1 (19-134), His Tag (PD1-H52H3) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Tyr 134 (Accession # Q9NZQ7-1).
Predicted N-terminus: Phe 19

This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.2 kDa. The protein migrates as 17 kDa and 19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
PD1-H52H3 works best for experiments that test the binding between PD-L1 and candidate antibodies, such as biopanning and other relevant assays.
This product is NOT suitable for testing PD1-PDL1 binding. For this type of application, we strongly recommend you to choose PD1-H82F3 as an alternative.
Less than 1.0 EU per μg by the LAL method / rFC method.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.