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Product Details
Synonyms
Atrial natriuretic peptide receptor 1, NPR1, NPRA, GC-A, ANPRA, NPR-A
Source
Mouse NPR1 Protein, His Tag (NP1-M52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 29 - Glu 469 (Accession # P18293-1).
Predicted N-terminus: Ser 29
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 54.1 kDa. The protein migrates as 65-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse NPR1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse NPR1 Protein, His Tag (Cat. No. NP1-M52H3) is more than 95% and the molecular weight of this protein is around 110-150 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Mouse NPR1 Protein, His Tag (Cat. No. NP1-M52H3) at 5 μg/mL (100 μL/well) can bind Mouse NPRA/NPR1 Antibody, Rat IgG2A with a linear range of 0.01-0.156 μg/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Natriuretic peptide receptor 1
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
7 Details
- Highest Development Stage：
Approved
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