Collect Product
Product Details
Synonyms
NOTCH1, Notch 1, hN1, TAN1
Source
Human NOTCH1, Fc Tag (NO1-H5255) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Gln 526 (Accession # P46531-1).
Predicted N-terminus: Ala 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 80.0 kDa. The protein migrates as 90-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Kunden haben auch angesehen
Data Display
SDS-PAGE
Human NOTCH1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) is more than 85% and the molecular weight of this protein is around 170-210 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human DLL4, His Tag (Cat. No. DL4-H5227) at 5 μg/mL (100 μL/well) can bind Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) with a linear range of 1-8 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human Jagged 1, His Tag (Cat. No. JA1-H52H9) immobilized on CM5 Chip can bind Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) with an affinity constant of 5.13 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) on Protein A Biosensor, can bind Human DLL4, His Tag (Cat. No. DL4-H5227) with an affinity constant of 67.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) on Protein A Biosensor, can bind Human DLL1 Protein, His Tag, premium grade (Cat. No. DL1-H52H8) with an affinity constant of 290 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Neurogenic locus notch homolog protein 1
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
1 Details
- Highest R&D Stage：
Phase 1 Clinical
Contact Us
Drug Candidate Licensing
