Product Details
Synonyms
CLEC5C, NKp80
Source
Alexa Fluor 647-Labeled Human NKp80 Protein, His Tag (NK0-HA243) is expressed from human 293 cells (HEK293). It contains AA Leu 60 - Tyr 231 (Accession # Q9NZS2-1).
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 23.9 kDa and 15.5 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE

Alexa Fluor 647-Labeled Human NKp80 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA

Immobilized Alexa Fluor 647-Labeled Human NKp80 Protein, His Tag (Cat. No. NK0-HA243) at 1 μg/mL (100 μL/well) can bind Human NKp80/KLRF1 Antibody, Rabbit IgG with a linear range of 0.6-20 ng/mL (QC tested).
Protocol
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