Product Details
Synonyms
NA, Neuraminidase
Source
Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag (NEE-V5245) is expressed from human 293 cells (HEK293). It contains AA Tyr 33 - Leu 466 (Accession # EPI_ISL_983345, GISAID).
Predicted N-terminus: His
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 55.7 kDa. The protein migrates as 65-80 kDa and 140 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a tetramer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Data Display
SDS-PAGE

Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5245) is more than 85% and the molecular weight of this protein is around 260-290 kDa verified by SEC-MALS.
Report
Bioactivity-SPR

a-Neu5Ac-PAA-biotin immobilized on SA Chip can bind Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5245) with an affinity constant of 59.7 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Influenza B [Austria/1359417/2021] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V5245) immobilized on CM5 Chip can bind N-Acetylneuraminic Acid (NANA,Neu5Ac) with an affinity constant of 0.518 mM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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