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Human Mucin-1 / MUC-1 (1036-1155) Protein, His Tag (Mass Spec verified)

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MU1-H52H5-100ug
$360.00
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MU1-H52H5-1mg
$2,615.00
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MU1-H52H5
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

Mucin 1, MUC1, CD227, EMA, H23AG, KL-6, MAM6, MUC-1, SEC, MUC-1, X, MUC1, ZD, PEM, PEMT, PUM, CA15-3, Episialin

Source

Human Mucin-1 (1036-1155), His Tag (MU1-H52H5) is expressed from human 293 cells (HEK293). It contains AA Leu 1036 - Gly 1155 (Accession # P15941-1).

Predicted N-terminus: Leu 1036 (partial α chain) & Ser 1098 (partial β chain)

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus. The mature form of Mucin-1 is a non-covalent heterodimeric complex with the proteolytically cleaved partial α and partial β chain. Each partial α and partial β chain has a calculated MW of 7.2 kDa (partial α chain) and 8.2 kDa (partial β chain). The protein migrates as 8 kDa (partial α chain) and 10-11 kDa (partial β chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Mass Spectrometry

  • Sequence Coverage

    Human Mucin-1 (1036-1155), His Tag(MU1-H52H5) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving chain1: 100% and chain2: 100% sequence coverage (Routinely tested).

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

Membrane mucins have several functions in epithelial cells including cytoprotection, extravasation during metastases, maintenance of luminal structure, and signal transduction. MUC17, contains an extended, repetitive extracellular glycosylation domain and a carboxyl terminus with two EGF-like domains, a SEA module domain, a transmembrane domain, and a cytoplasmic domain with potential serine and tyrosine phosphorylation sites. Interacts via its C-terminus with PDZK1 and this interaction appears important for proper localization. Probably plays a role in maintaining homeostasis on mucosal surfaces.