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Product Details
Synonyms
MDK, MEK, Midkine, MK1, MKARAP, NEGF2, Retinoic acid-induced differentiation factor
Source
Human Midkine Protein, premium grade (MIE-H5117) is expressed from E. coli cells. It contains AA Val 21 - Asp 143 (Accession # P21741-1).
Predicted N-terminus: Met 1
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 13.6 kDa. The protein migrates as 16-17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Sterility
Negative
Mycoplasma
Negative
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human Midkine Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Human Midkine Protein, premium grade (Cat. No. MIE-H5117) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human Midkine Protein, premium grade (Cat. No. MIE-H5117) at 2 μg/mL (100 μL/well) can bind Human Glypican 2, His Tag (Cat. No. GP2-H52H3) with a linear range of 1-63 ng/mL (QC tested).
Protocol
Immobilized Human Midkine Protein, premium grade (Cat. No. MIE-H5117) at 2 μg/mL (100 μL/well) can bind Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) with a linear range of 1-31 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human Midkine Protein, premium grade (Cat. No. MIE-H5117) immobilized on CM5 Chip can bind Human Glypican 2, His Tag (Cat. No. GP2-H52H3) with an affinity constant of 44.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Midkine
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
1 Details
- Highest R&D Stage：
Clinical
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