Collect Product
Product Details
Synonyms
MSLN, Mesothelin, MPF
Source
Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling (MSN-H826x) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272). It is the biotinylated form of Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H526x).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 59.1 kDa. The protein migrates as 60-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Kunden haben auch angesehen
Data Display
SDS-PAGE
Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling (Cat. No. MSN-H826x) is more than 85% and the molecular weight of this protein is around 125-153 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Anti-Human MSLN Mab at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling (Cat. No. MSN-H826x) with a linear range of 0.5-8 ng/mL (QC tested).
Protocol
Evaluation of CAR expression
FACS Analysis of Anti-MSLN CAR Expression
293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling (Cat. No. MSN-H826x, 3 µg/ml) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling (Cat. No. MSN-H826x).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Mesothelin
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
55 Details
- Highest R&D Stage：
Phase 2 Clinical
Contact Us
Drug Candidate Licensing
