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Product Details
Synonyms
M-CSF, CSF-1, Lanimostim
Source
Biotinylated Human M-CSF, His,Avitag (MCF-H82E6) is expressed from human 293 cells (HEK293). It contains AA Glu 33 - Arg 255 (Accession # P09603-1).
Predicted N-terminus: Glu 33
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 28.8 kDa. The protein migrates as 35-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Human M-CSF, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Human M-CSF R, Fc Tag (Cat. No. CSR-H5258) at 2 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Cynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) with a linear range of 1-39 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) immobilized on SA Chip can bind Human M-CSF R, His Tag (Cat. No. CSR-H5228) with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) on SA Biosensor, can bind Human M-CSF R, Fc Tag, low endotoxin (Cat. No. CSR-H5258) with an affinity constant of 24.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-CELL BASE
Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) stimulates proliferation of RAW264.7 cells. The EC50 for this effect is 6.20-7.15 ng/mL (Routinely tested).
Protocol
The bio-activity of Biotinylated Human M-CSF / CSF-1 Protein, His,Avitag™ (Cat. No. MCF-H82E6) was determined by dose-dependent stimulation of the proliferation of M-NFS-60 cells. The EC50 for this effect is 10.91ng/mL (Routinely tested).
Protocol
User Reviews Publish Comment
FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Macrophage Colony Stimulating Factor 1
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
0 Details
- Highest R&D Stage：
Preclinical
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