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Product Details
Synonyms
LTBR, D12S370, TNFCR, TNFR3, TNFRSF3, TNFRIII
Source
Mouse LTBR Protein, His Tag (LTR-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Leu 223 (Accession # P50284).
Predicted N-terminus: Gln 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.6 kDa. The protein migrates as 28-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse LTBR Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse LTBR Protein, His Tag (Cat. No. LTR-M52H3) is more than 90% and the molecular weight of this protein is around 23-35 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Mouse LTBR Protein, His Tag (Cat. No. LTR-M52H3) at 5 μg/mL (100 μL/well) can bind Human LIGHT Protein, Fc Tag (Cat. No. LIT-H5269) with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Mouse LTBR Protein, His Tag (Cat. No. LTR-M52H3) immobilized on CM5 Chip can bind Human LIGHT Protein, Fc Tag (Cat. No. LIT-H5269)with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 3
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 1 Clinical
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