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Product Details
Synonyms
LRP5, LRP-5, LRP-7, LRP7, LR3
Source
Biotinylated Human LRP-5, His,Avitag (LR5-H82E6) is expressed from human 293 cells (HEK293). It contains AA Glu 644 - Gln 1263 (Accession # O75197-1 ).
Predicted N-terminus: Glu 644
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 73.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
Biotin-labeled
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
0.7-1.0 as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human LRP-5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) is more than 90% and the molecular weight of this protein is around 70-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human SOST, His Tag (Cat. No. HST-H5245) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) with a linear range of 0.078-1.25 μg/mL (Routinely tested).
Protocol
Immobilized Human Dkk-1, His Tag (Cat. No. DK1-H5221) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) with a linear range of 0.078-1.25 μg/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) on SA Biosensor, can bind Human SOST, His Tag (Cat. No. HST-H5245) with an affinity constant of 7.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) on SA Biosensor, can bind Human Dkk-1, His Tag (Cat. No. DK1-H5221) with an affinity constant of 93.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Low-density lipoprotein receptor-related protein 5
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 3 Clinical
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