Human L-Selectin / CD62L Protein, His Tag

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SEL-H52H3-100ug
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SEL-H52H3-1mg (500ug X 2)
$2,615.00
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SEL-H52H3
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  • Product Details
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Synonyms

SELL, CD62L, LNHR, LYAM1, L-selectin, LAM-1, LECAM1, TQ1, gp90-MEL

Source

Human L-Selectin Protein, His Tag (SEL-H52H3) is expressed from human 293 cells (HEK293). It contains AA Trp 39 - Asn 332 (Accession # P14151-1).

Predicted N-terminus: Trp 39

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 39.2 kDa. The protein migrates as 47-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

L-selectin (CD62L) is a type-I transmembrane glycoprotein and cell adhesion molecule that is expressed on most circulating leukocytes. Since its identification in 1983, L-selectin has been extensively characterized as a tethering/rolling receptor. There is now mounting evidence in the literature to suggest that L-selectin plays a role in regulating monocyte protrusion during transendothelial migration (TEM).