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Human ITPRIPL1 Protein, Fc Tag (MALS verified)

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Product Number/Spec
Price
Menge
IT1-H5253-100ug
$385.00
IT1-H5253-1mg
$2540.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    KIAA1754L

  • Source

    Human ITPRIPL1 Protein, Fc Tag (IT1-H5253) is expressed from human 293 cells (HEK293). It contains AA His 25 - Gly 103 (Accession # Q6GPH6).

    Predicted N-terminus: His 25

    Request for sequence
  • Molecular Characterization

    ITPRIPL1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 35.7 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    ITPRIPL1 SDS-PAGE

    Human ITPRIPL1 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    ITPRIPL1 SEC-MALS

    The purity of Human ITPRIPL1 Protein, Fc Tag (Cat. No. IT1-H5253) is more than 85% and the molecular weight of this protein is around 68-88 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     ITPRIPL1 ELISA

    Immobilized Human ITPRIPL1 Protein, Fc Tag (Cat. No. IT1-H5253) at 1 μg/mL (100 μL/well) can bind ITPRIPL1 antibody with a linear range of 0.001-0.125 μg/mL (QC tested).

    Protocol

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Publish Comment

Background Introduction

Inositol 1,4,5-triphosphate receptor interacting protein like 1 (ITPRIPL1) is a single transmembrane protein,exhibits normal testis-enriched expression and widespread overexpression in various human cancers. ITPRIPL1 is commonly found in tumors with low PD-L1 expression. ITPRIPL1 acts as a natural ligand for CD3 ε, evading immune surveillance, leading to decreased T cell activity and promoting tumor growth. Research has shown that inducing stable CD3E-NCK1 binding prevents CD3E-ZAP70 interaction, subsequently inhibiting downstream ERK-NFkB signaling cascade and calcium influx activation.

Frontier Progress

 
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