Human Integrin alpha D beta 2 (ITGAD&ITGB2) Heterodimer Protein, His Tag&Tag Free

Synonyms

Integrin alpha D beta 2, ITGAD & ITGB2

Source

Human ITGAD&ITGB2 Heterodimer Protein, His Tag&Tag Free (IT2-H53W6) is expressed from CHO cells. It contains AA Phe 18 - Asn 1099 (ITGAD) & Gln 23 - Asn 700 (ITGB2) (Accession # Q13349-1 (ITGAD) & P05107-1 (ITGB2) (H354Q)).

Predicted N-terminus: Phe 18 (ITGAD) & Gln 23 (ITGB2)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Human Integrin alpha D beta 2 (ITGAD&ITGB2) Heterodimer Protein, produced by co-expression of ITGAD and ITGB2, has a calculated MW of 124.9 kDa (ITGAD) and 80.2 kDa (ITGB2). Subunit ITGAD is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 150 kDa (ITGAD) and 90-100 kDa (ITGB2) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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