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Product Details
Synonyms
IL5Ra, CD125
Source
Mouse IL-5 R alpha Protein, His Tag (ILA-M52H7) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 339 (Accession # P21183).
Predicted N-terminus: Asp 18
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 38.7 kDa. The protein migrates as 50-58 kDa and 75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse IL-5 R alpha Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse IL-5 R alpha Protein, His Tag (Cat. No. ILA-M52H7) is more than 90% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Mouse IL-5 R alpha Protein, His Tag (Cat. No. ILA-M52H7) immobilized on CM5 Chip can bind Mouse IL-5, His Tag (Cat. No. IL5-M52H3) with an affinity constant of 243 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-5 receptor subunit alpha
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Approved
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