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Human IL-36 alpha / IL-1F6 Protein, Tag Free (MALS & SPR verified)

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Product Number/Spec
Price
Menge
IL6-H5117-100ug
$310.00
IL6-H5117-1mg (500ug X 2)
$2245.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    FIL1 epsilon, IL-1 epsilon, IL-1F6, IL36A, FIL1E, IL1E, IL1F6

  • Source

    Human IL-36 alpha Protein, Tag Free (IL6-H5117) is expressed from E. coli cells. It contains AA Lys 6 - Phe 158 (Accession # Q9UHA7).

    Predicted N-terminus: Lys

    Request for sequence
  • Molecular Characterization

    IL-36 alpha Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 17.1 kDa. The protein migrates as 15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    IL-36 alpha SDS-PAGE

    Human IL-36 alpha Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-36 alpha SEC-MALS

    The purity of Human IL-36 alpha Protein, Tag Free (Cat. No. IL6-H5117) is more than 95% and the molecular weight of this protein is around 17-25 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     IL-36 alpha SPR

    Biotinylated Human IL-1 Rrp2, His,Avitag (Cat. No. IL2-H82E9) immobilized on SA Chip can bind Human IL-36 alpha Protein, Tag Free (Cat. No. IL6-H5117) with an affinity constant of 373 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Publish Comment

Background Introduction

The IL-36 subfamily of cytokines is the most diverse, including three agonists(IL-36α, IL-36β, and IL-36γ), one antagonist(IL-36Ra), one cognate receptor(IL-36R) and one accessory protein(IL-1RAcP). The receptor activation follows a two-step mechanism in that the agonist first binds to IL-36R and the resulting binary complex recruits IL-1RAcP. IL-36α that binds to and signals through the IL-36R which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Appropriately balanced IL-36 signaling is a critical determinant of skin and lung health.

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