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IL33, DV27, C9ORF26, IL1F11, NFHEV, DKFZp586H0523, DVS27, NFEHEV, RP11-575C20.2
Human IL-33 (C208S, C227S, C232S, C259S) Protein, His Tag (IL3-H52H6) is expressed from human 293 cells (HEK293). It contains AA His 109 - Thr 270 (Accession # O95760-1(C208S,C227S,C232S,C259S)).
Predicted N-terminus: His 109
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Human IL-33 (C208S, C227S, C232S, C259S) Protein, His Tag(IL3-H52H6) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 99.5% sequence coverage (Routinely tested).
Less than 0.01 EU per μg by the LAL method / rFC method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
