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Product Details
Synonyms
Interleukin-27, IL27A&IL27B, EBI3&p28
Source
Human IL-27 Protein, premium grade (IL7-H5319) is expressed from CHO cells. It contains AA Phe 29 - Pro 243 & Arg 21 - Lys 229 (Accession # Q8NEV9-1 & Q14213-1).
Predicted N-terminus: Phe 29
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 47.8 kDa. The protein migrates as 54 kDa±3 kDa, 78 kDa and 166 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Host Cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Negative
Mycoplasma
Negative
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.0 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human IL-27 Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-CELL BASE
Human IL-27 Protein, premium grade (Cat. No. IL7-H5319) stimulates STAT3 (Luc) HEK293 Reporter Cells. The specific activity of Human IL-27 Protein, premium grade is > 1.00 x 10^4 U/mg (QC tested).
Protocol
Bioactivity-ELISA
Immobilized Human IL-27 Protein, premium grade (Cat. No. IL7-H5319) at 2 μg/mL (100 μL/well) can bind Human IL-27 Ra, Fc Tag (Cat. No. ILA-H5254) with a linear range of 1-20 ng/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-27
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
1 Details
- Highest Development Stage：
Phase 2 Clinical
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