Human IL-22BP Protein, His Tag

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Cat. No. / Size
Price
Qty
ILP-H52H3-100ug
$360.00
ILP-H52H3-1mg
$2380.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IL22RA2, CRF2-S1, IL-22BP, CRF2-10, CRF2-S1

  • Source

    Human IL-22BP Protein, His Tag (ILP-H52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Pro 231 (Accession # Q969J5-2).

    Predicted N-terminus: Thr 22

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  • Molecular Characterization

    IL-22BP Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.6 kDa. The protein migrates as 42-52 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-22BP SDS-PAGE

    Human IL-22BP Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     IL-22BP ELISA

    Immobilized Human IL-22BP Protein, His Tag (Cat. No. ILP-H52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) with a linear range of 1-39 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     IL-22BP SPR

    Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) captured on Biotin CAP-Series S Sensor Chip can bind Human IL-22BP Protein, His Tag (Cat. No. ILP-H52H3) with an affinity constant of 0.162 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Background

This gene encodes a member of the class II cytokine receptor family. The encoded soluble protein specifically binds to and inhibits interleukin 22 activity by blocking the interaction of interleukin 22 with its cell surface receptor. The encoded protein may be important in the regulation of inflammatory response, and has been implicated in the regulation of tumorigenesis in the colon. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Isoform 1 may play a role in establishing and maintaining successful pregnancy. Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.

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