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Product Details
Synonyms
IL-22 R alpha 1 & IL-10 R beta
Source
Human IL-22 R alpha 1 & IL-10 R beta Heterodimer Protein, Fc Tag&Fc Tag (ILA-H525k) is expressed from human 293 cells (HEK293). It contains AA Pro 18 - Thr 228 & Met 20 - Ser 220 (Accession # Q8N6P7-1 & Q08334).
Predicted N-terminus: Pro 18 & Met 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human IL-22 R alpha 1 & IL-10 R beta Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of IL-22 R alpha 1 and IL-10 R beta, has a calculated MW of 50.6 kDa & 49.7 kDa. Subunit IL-22 R alpha 1 is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-10 R beta is fused with a human IgG1 Fc tag at the C-terminus. The protein migrates as 63-93 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IL-22 R alpha 1 & IL-10 R beta Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-22 R alpha 1 & IL-10 R beta Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILA-H525k) is more than 90% and the molecular weight of this protein is around 105-135 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-22 R alpha 1 & IL-10 R beta Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILA-H525k) with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
Immobilized Human IL-22, His Tag (Cat. No. IL2-H524a) at 5 μg/mL (100 μL/well) can bind Human IL-22 R alpha 1 & IL-10 R beta Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILA-H525k) with a linear range of 0.6-20 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human IL-22 R alpha 1 & IL-10 R beta Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILA-H525k) captured on Protein A Chip can bind Human IL-22, His Tag (Cat. No. IL2-H524a) with an affinity constant of 0.301 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human IL-22 R alpha 1 & IL-10 R beta Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILA-H525k) captured on Protein A Chip can bind Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) with an affinity constant of 0.400 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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