Human IL-22 R alpha 1 & IL-10 R beta Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

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ILA-H525k-100ug
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ILA-H525k-1mg (500ug X 2)
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Synonyms

IL-22 R alpha 1 & IL-10 R beta

Source

Human IL-22 R alpha 1 & IL-10 R beta Heterodimer Protein, Fc Tag&Fc Tag (ILA-H525k) is expressed from human 293 cells (HEK293). It contains AA Pro 18 - Thr 228 & Met 20 - Ser 220 (Accession # Q8N6P7-1 & Q08334).

Predicted N-terminus: Pro 18 & Met 20

Molecular Characterization

Human IL-22 R alpha 1 & IL-10 R beta Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of IL-22 R alpha 1 and IL-10 R beta, has a calculated MW of 50.6 kDa & 49.7 kDa. Subunit IL-22 R alpha 1 is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-10 R beta is fused with a human IgG1 Fc tag at the C-terminus. The protein migrates as 63-93 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a heterodimer.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background Introduction

Interleukin-22 receptor subunit alpha-1(IL22RA1) is a component of the receptor for IL20, IL22 and IL24.Component of IL22 receptor formed by IL22RA1 and IL10RB enabling IL22 signaling via JAK/STAT pathways. IL22 also induces activation of MAPK1/MAPK3 and Akt kinases pathways. Component of one of the receptor for IL20 and IL24 formed by IL22RA1 and IL20RB also signaling through STATs activation. Mediates IL24 antiangiogenic activity as well as IL24 inhibitory effect on endothelial cell tube formation and differentiation.