Product Details
Synonyms
IL-18, Interleukin-18, IL18, IL-1 gamma, IGIF, IL1F4, IL-1F4
Source
Mouse IL-18 Protein, His Tag (IL8-M51H3) is expressed from E. coli cells. It contains AA Asn 36 - Ser 192 (Accession # P70380).
Predicted N-terminus: Asn
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.0 kDa. The protein migrates as 20-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE

Mouse IL-18 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA

Immobilized Recombinant Mouse IL-18 BPd Fc Chimera Protein, CF at 5 μg/mL (100 μL/well) can bind Mouse IL-18 Protein, His Tag (Cat. No. IL8-M51H3) with a linear range of 0.2-10 ng/mL (QC tested).
Protocol
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