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Product Details
Synonyms
IL-17F, Interleukin-17F, Cytokine ML-1
Source
Biotinylated Human IL-17F, His,Avitag (ILF-H82Q9) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 18.5 kDa. The protein migrates as 25-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Human IL-17F, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) is more than 95% and the molecular weight of this protein is around 37-55 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) with a linear range of 0.6-78 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116)precoated (0.5 μg/well) plate can bind Human IL-17 RA, Fc Tag (MALS verified) with a linear range of 1-78 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-17 RC, Fc Tag with a linear range of 2-156 ng/mL (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Interleukin-17F
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
5 Details
- Highest R&D Stage：
NDA/BLA
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