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Product Details
Synonyms
IL-17A, Interleukin-17A, CTLA-8, IL-17
Source
Canine IL-17A, His Tag (ILA-C52H7) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Ala 155 (Accession # NP_001159350.1).
Predicted N-terminus: Gly 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 17.0 kDa. The protein migrates as 15 kDa and 20-23 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Canine IL-17A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Canine IL-17A, His Tag (Cat. No. ILA-C52H7) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Canine IL-17A, His Tag (Cat. No. ILA-C52H7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL17A Antibody, Human IgG1 with a linear range of 0.06-4 ng/mL (QC tested).
Protocol
Immobilized Human IL-17 RA Protein, Fc Tag (Cat. No. ILA-H525f) at 2 μg/mL (100 μL/well) can bind Canine IL-17A, His Tag (Cat. No. ILA-C52H7) with a linear range of 0.0006-0.156 μg/mL (Routinely tested).
Protocol
Immobilized Human IL-17 RC (21-465) Protein, Fc Tag (Cat. No. ILC-H5258) at 2 μg/mL (100 μL/well) can bind Canine IL-17A, His Tag (Cat. No. ILA-C52H7) with a linear range of 0.001-0.078 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin 17A
- Category：
- Approved Drugs：
5 Details
- Drugs in Clinical Trials：
35 Details
- Highest Development Stage：
Approved
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