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Product Details
Synonyms
IL-11, Interleukin-11, AGIF, Oprelvekin, IL11
Source
Canine IL-11 Protein, His Tag (IL1-C5246) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Leu 199 (Accession # A0A8C0QKD2).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 21 kDa. The protein migrates as 23-24 kDa and 26-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 10 mM Citric acid, PBS, pH3.0 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Canine IL-11 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Canine IL-11 Protein, His Tag (Cat. No. IL1-C5246) is more than 90% and the molecular weight of this protein is around 22-42 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-11 R alpha, Fc,Avitag (Cat. No. ILR-H82F5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Canine IL-11 Protein, His Tag (Cat. No. IL1-C5246) with a linear range of 2-31 ng/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-11
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Approved
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