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Human IGFBP-1 Protein, His Tag (MALS verified)

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Product Number/Spec
Price
Menge
IG1-H52H3-100ug
$310.00
IG1-H52H3-1mg (500ug X 2)
$2040.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    IGFBP1, PP12, IBP1

  • Source

    Human IGFBP-1 Protein, His Tag (IG1-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Asn 259 (Accession # P08833).

    Predicted N-terminus: Ala 26

    Request for sequence
  • Molecular Characterization

    IGFBP-1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.2 kDa. The protein migrates as 32-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    IGFBP-1 SDS-PAGE

    Human IGFBP-1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IGFBP-1 SEC-MALS

    The purity of Human IGFBP-1 Protein, His Tag (Cat. No. IG1-H52H3) is more than 95% and the molecular weight of this protein is around 22-32 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IGFBP-1 ELISA

    Immobilized Human IGF-I, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IGFBP-1 Protein, His Tag (Cat. No. IG1-H52H3) with a linear range of 0.01-0.156 μg/mL (QC tested).

    Protocol

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Publish Comment

Background Introduction

IGFBP1 stands for insulin-like growth factor binding protein 1. It is a protein that binds to insulin-like growth factors (IGFs) and regulates their availability and activity within the body. IGFBP1 is part of a family of six IGFBPs (IGFBP1-6) that interact with IGFs to modulate their biological effects. IGFBP1 binds to IGFs (mostly IGF-1) with high affinity and prolongs their half-life in the circulation by protecting them from degradation. IGFBP1 has also been found to have IGF-independent functions, suggesting that it may have additional roles beyond regulating IGF signaling.

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