Unconjugated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Complex Protein (Monomer, MALS verified)

Synonyms

HLA-A*0201 & B2M & WT-1 (RMFPNAPYL)

Source

Unconjugated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Complex Protein (HL1-H82Eb) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & RMFPPYL peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769 (B2M) & RMFPPYL).

Predicted N-terminus: Gly 25 & Ile 21

Molecular Characterization

Unconjugated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Complex Protein is produced by co-expression of HLA-A*02:01 and B2M loaded with WT-1 peptide.

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™), and it is not biotinylated.

The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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