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Product Details
Synonyms
ERBB2, CD340, HER-2, neu, HER2, MLN19, NEU, NGL, TKR1
Source
Human Her2, His Tag (HE2-H5225) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
Predicted N-terminus: Thr 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 70.2 kDa. The protein migrates as 70-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.2 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human Her2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Her2, His Tag (Cat. No. HE2-H5225) is more than 95% and the molecular weight of this protein is around 75-105 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL (100 μL/well) can bind Anti-Her2 antibody with a linear range of 0.061-1.95 ng/mL (QC tested).
Protocol
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL (100 μL/well) can bind Pertuzumab Biosimilar with a linear range of 0.2-4 ng/mL (Routinely tested).
Protocol
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL (100 μL/well) can bind Trastuzumab Biosimilar with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL (100 μL/well) can bind Bispecific Antibody (Her2 × Her3) with a linear range of 0.8-3 ng/mL (Routinely tested).
Protocol
Bioactivity-TR-FRET
In this TR-FRET assay, Monoclonal Anti-Human IgG Antibody Europium chelate (for TR-FRET) (Cat. No. FRT-TD02) is used as the Donor and Monoclonal Anti-His Tag Antibody, Rabbit IgG FA-Labeled (for TR-FRET) (Cat. No. FRT-TA11) is used as the Acceptor. Human Her2, His Tag (Cat. No. HE2-H5225), at 1.25 ng/well, can bind to the Disitamab with a linear detection range of 2-0.002 μg/mL (Routinely tested).
Bioactivity-SPR
Herceptin (Trastuzumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Her2, His Tag (Cat. No. HE2-H5225) with an affinity constant of 1.07 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Herceptin (Trastuzumab) on AHC Biosensor, can bind Human Her2, His Tag (Cat. No. HE2-H5225) with an affinity constant of 0.825 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
G***z2025-03-12
LikesWe bought the Her2 to perform SPR to determine the binding affinity of in house produced herceptin. Using the His tag it was easy to capture the protein with amine coupled anti His antibody on the sensor chip surface. Therefore we could prove our antibody has full binding functionality very easily using the biacore T200 system. Ordering and shipping was really simple and all the necessary documentation can be found online. Although I didn't find the CoA in the first place everything was described easily and protocols of different analyses were available.
- 1***22019-10-12 LikesThe Her-2 protein has good bioactivity and can be detected by ELISA with a low coating concentration. The amount we used was small so it was cost-effective. I also bought another antigen which also showed really good bioactivity. Customer service is also excellent.
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Receptor protein-tyrosine kinase erbB-2
- Category：
- Listed Drugs Count：
47 Details
- Clinical Drugs Count：
238 Details
- Highest R&D Stage：
Approved
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