Human Her2 & ErbB3 Protein, Fc Tag&Fc Tag (MALS verified)

Synonyms

Her2 & ErbB3, HER2 & HER3

Source

Human Her2 & ErbB3 Protein, Fc Tag&Fc Tag (HE2-H5254) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 & Ser 20 - Thr 643 (Accession # P04626 & P21860).

Predicted N-terminus: Thr 23 & Ser 20

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Human Her2 & ErbB3 Protein, Fc Tag&Fc Tag, produced by co-expression of HER2 and ErbB3, has a calculated MW of 95.9 kDa (HER2) and 94.9 kDa (ErbB3). Subunit HER2 is fused with a human IgG1 Fc fragment at the C-terminus and subunit ErbB3 is fused with a human IgG1 Fc fragment at the C-terminus. The protein migrates as 110-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process